Expression of Kyphosis in Young Pigs is Induced by a Reduction of Supplemental Vitamin D in Maternal Diets and Vitamin D, Ca, and P Concentrations in Nursery Diets

October 31, 2012 — 

Kyphosis is an idiopathic disease characterized by abnormal, outward spinal curvature. A spontaneous outbreak and subsidence of kyphosis over a 4-mo period in the University of Wisconsin Swine Research and Teaching Center herd coincided with an accidental omission of vitamin D(3) in 1 of 2 premixes used in sow diets. This experiment was designed as a controlled study to confirm that vitamin D deletion from the premix used previously in sow diets would induce kyphosis in their offspring. Crossbred (Landrace × Large White), multiparious sows (n = 8) were fed corn-SBM diets supplemented with either 325 (+D) or 45 (-D) IU vitamin D3/kg diet from breeding through lactation. The vitamin D concentrations duplicated formulations of diets fed during the earlier spontaneous outbreak. At weaning (approximately 4 wk), pigs were fed diets devoid of supplemental vitamin D and formulated to supply either 120% (HCaP) or 80% (LCaP) of required Ca and P concentrations until wk 9. At wk 9, all pigs were fed the HCaP diet until wk 13. No evidence of kyphosis was observed in pigs at weaning. Pigs produced by -D sows and fed LCaP diets exhibited a 17% incidence (4/23 pigs) of kyphosis at wk 9. At wk 13, the incidence of kyphosis had increased to 32% (6/19 pigs). Unexpectedly at wk 13, pigs produced by +D sows and fed LCaP diets exhibited a 26% incidence (5/19 pigs) of kyphosis. None of the pigs fed HCaP diets from wk 4 to 13 displayed kyphosis, regardless of maternal diets. Evidence of kyphosis was detected at a younger age if pigs were produced by sows fed -D diets. Whole body and femur bone mineral content determined by dual energy X-ray absorptiometry were reduced (P < 0.05) in pigs fed LCaP vs. HCaP diets, but pigs produced by -D sows were more severely affected. Femur bending moments were reduced (P < 0.05) at wk 9 and 13 in pigs fed LCaP vs. HCaP diets. At wk 13, pigs produced by -D sows and fed LCaP diets had reduced (P < 0.05) bone mineral density and femur yield bending moment compared with pigs from +D sows fed LCaP diets. In conclusion, the 20 to 30% incidence of kyphosis induced by altering vitamin D, Ca, and P concentrations in maternal and nursery diets mimics the incidence observed in spontaneous outbreaks in afflicted herds. A reproducible vitamin D-induced kyphosis in young pigs offers a suitable model to study the skeletal tissue characteristics, fetal skeletal tissue development, and potential treatments for pigs and human patients afflicted by this disease.

Rortvedt LA, Crenshaw TD. Expression of Kyphosis in Young Pigs is Induced by a Reduction of Supplemental Vitamin D in Maternal Diets and Vitamin D, Ca, and P Concentrations in Nursery Diets; J Anim Sci. 2012 Oct 16. [Epub ahead of print] http://www.ncbi.nlm.nih.gov/pubmed/23100590