Abstract: Pluck-pools as diagnostic samples for detecting porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in porcine abortion material and stillbirths
May 10, 2024 —
Abstract: Pluck-pools as diagnostic samples for detecting porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in porcine abortion material and stillbirths
Investigating infectious agents in porcine abortion material and stillborn piglets poses challenges for practitioners and diagnostic laboratories. In this study, pooled samples of individual reference organs (thymus and heart) from a total of 1000 aborted fetuses and stillborn piglets were investigated using quantitative PCR protocols for porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) and porcine circovirus type 2 (PCV2). Simultaneously, a pluck-pool containing equivalent portions of fetal thymus, heart, and lung tissue was collected, frozen at - 20 °C, and re-analyzed when a certain amount of either PRRSV-1 RNA or PCV2 DNA was detected in individual reference organs. Thirteen pluck-pools were assessed for PRRSV-1, all being PCR-positive. For PCV2, 11 of 15 pluck-pools investigated were PCR-positive. In all pluck-pools testing negative, viral loads in individual pools were low. This study indicates that pluck-pools can be valuable diagnostic material and the consolidation of multiple organs through a single RNA/DNA extraction optimizes the utilization of available laboratory resources. Additional research is required to assess the feasibility of follow-up investigations and to accurately define criteria for interpretation of viral loads in a clinical context.
Kreutzmann H, Unterweger C, Schwarz L, Dimmel K, Auer A, Rümenapf T, Ladinig A. Pluck-pools as diagnostic samples for detecting porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in porcine abortion material and stillbirths. Vet J. 2024 Apr;304:106081. https://doi.org/10.1016/j.tvjl.2024.106081. Epub 2024 Feb 13. PMID: 38360136.
- Next story: Save the Date: AVMA’s DEIW Summit Nov 7-9
- Next in category: Abstract: Boar semen storage at 5 °C for the reduction of antibiotic use in pig insemination: Pathways from science into practice
- Previous in category: Abstract: Mapping the future of antimicrobial use data collection in US animal agriculture: insights from FDA-funded pilot studies
- Previous story: Position Announcement: Swine Veterinarian